Profiling of Cancer Cells via Phenotype Microarray AnalysisBy Nichole Wetton, ’14 Biology
The cell lines were treated with these six reagents in a dose dependent manner to determine whether the growth inhibition observed was due to targeting of the insulin/mTOR (mammalian Target of Rapamycin, a protein implicated in a wide variety of cancers) pathway. This was studied using an ELISA based assay called InstantOne ELISA which detects a specific protein in the mTOR pathway. It was observed that cancer cells from different tissues respond differently to the same chemical and that in some cell lines the mTOR pathway is in fact being targeted while in others it was not.
This research has been very exciting for me and it is amazing to see how cancer cells grow and behave compared to normal cells. The field of oncology is quite fascinating as there is no end to the limit of studying cancer cells. I hope to reap the benefits of this valuable research experience when I apply for a graduate degree program soon. I highly encourage all students to conduct research in some capacity as these experiential skills are truly gratifying, both at the personal and scientific level.